RNA-HISATIDE for Studying Gene Expression and Detection of Changes in RNA Stability

Target Name: RHBDF1

NCBI ID: G64285

RNA-HISATIDE for Studying Gene Expression and Detection of Changes in RNA Stability

RNA-HISATIDEs (ribonucleotide-hashed aggregated transcripts) are a type of gene expression assay that has been widely used to study gene expression and discovery. They are based on the principle of DNA-protein binding and use RNA-protein interactions to amplify the signal of specific gene expression. One of the most popular types of RNA-HISATIDEs is the high-throughput RNA-HISATIDE (RHDF1), which can be used to detect changes in gene expression in a wide variety of samples, including tissues, fluids, and cell lines.

The RHDF1 is a construct that is derived from the human genome and contains a unique 18-mer RNA fragment that is derived from the 5' end of the gene. This RNA fragment is synthesized using an limiting enzyme that creates a double-stranded RNA molecule that can be amplified using PCR. The RHDF1 construct is then introduced into cells or tissues, where it can be used to detect changes in gene expression by hybridizing with specific RNA probes.

One of the key features of the RHDF1 is its ability to detect changes in gene expression that occur at the level of RNA stability, rather than just changes in gene expression itself. This is achieved by using a unique assay system that allows the RHDF1 to detect changes in the stability of RNA molecules, as well as changes in their levels.

The RHDF1 construct is based on a specific protocol for generating RNA-HISATIDEs, which involves a combination of PCR, cloning, and amplification. The construct is designed to include a unique 18-mer RNA fragment that is derived from the 5' end of the gene, as well as a control region that is used to amplify the signal of the RHDF1 construct. The RHDF1 construct is then introduced into cells or tissues, where it can be used to detect changes in gene expression by hybridizing with specific RNA probes.

One of the key benefits of the RHDF1 is its high sensitivity and specificity for detecting changes in gene expression. This is achieved by using a unique assay system that allows the RHDF1 to detect changes in the stability of RNA molecules, as well as changes in their levels. The RHDF1 construct is based on a specific protocol for generating RNA-HISATIDEs, which involves a combination of PCR, cloning, and amplification. The construct is designed to include a unique 18-mer RNA fragment that is derived from the 5' end of the gene, as well as a control region that is used to amplify the signal of the RHDF1 construct.

The RHDF1 is a powerful tool for studying gene expression and can be used to detect changes in gene expression that occur at the level of RNA stability, as well as changes in their levels. This is achieved by using a unique assay system that allows the RHDF1 to detect changes in the stability of RNA molecules, as well as changes in their levels. The RHDF1 construct is based on a specific protocol for generating RNA-HISATIDEs, which involves a combination of PCR, cloning, and amplification. The construct is designed to include a unique 18-mer RNA fragment that is derived from the 5' end of the gene, as well as a control region that is used to amplify the signal of the RHDF1 construct.

Protein Name: Rhomboid 5 Homolog 1

Functions: Regulates ADAM17 protease, a sheddase of the epidermal growth factor (EGF) receptor ligands and TNF, thereby plays a role in sleep, cell survival, proliferation, migration and inflammation. Does not exhibit any protease activity on its own

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