POLR2J3: Role in DNA Replication & Cancer (G548644)

Target Name: POLR2J3

NCBI ID: G548644

POLR2J3: Role in DNA Replication & Cancer

POLR2J3, also known as DNA-directed RNA polymerase II subunit 11, is a protein that plays a crucial role in the process of DNA replication in eukaryotic cells. It is a subunit of the DNA-directed RNA polymerase II enzyme, which is responsible for creating double-stranded RNA from single-stranded DNA during the process of transcription.

POLR2J3 is a 23-kDa protein that consists of 1,312 amino acids. It has a molecular weight of 196 kDa and a calculated pI of 6.5. The protein has a characteristic Rossmann-fold structure, which is a type of hydrogen bonding motif that is commonly found in proteins that involve DNA-binding.

POLR2J3 is expressed in most eukaryotic cells and is involved in the early stages of DNA replication. It is highly conserved across different species, with only minor differences in its sequence.

One of the key functions of POLR2J3 is its ability to bind to the double-stranded DNA template. It has been shown to bind to DNA in a process called \"complex formation,\" where it recognizes the target DNA sequence and forms a stable complex with it. This interaction between the protein and DNA is essential for the efficient initiation of DNA replication.

In addition to its role in DNA replication, POLR2J3 has also been shown to play a role in the regulation of gene expression. It has been shown to interact with the protein RNA polymerase II, which is involved in the process of transcription. This interaction allows the protein to regulate the amount of RNA produced from a particular gene.

POLR2J3 has also been shown to be involved in the process of DNA damage repair. When DNA double-strands are damaged, the protein is responsible for repairing the damage and ensuring that the double-stranded DNA is restored. This process is important for maintaining the integrity of genetic material and is a critical step in the DNA replication process.

POLR2J3 is also a potential drug target in cancer. Studies have shown that high levels of POLR2J3 are associated with poor prognosis in various types of cancer, including breast, ovarian, and colorectal cancers. Additionally, some studies have shown that inhibiting the activity of POLR2J3 has anticancer effects.

Furthermore, POLR2J3 has also been shown to be involved in the process of post-transcriptional modification (PTM), which is a process by which RNA molecules are modified after they have been synthesized but before they are translated into proteins. This modification process plays a crucial role in the regulation of gene expression and has been implicated in various diseases, including cancer.

In conclusion, POLR2J3 is a protein that plays a crucial role in the process of DNA replication in eukaryotic cells. Its ability to bind to double-stranded DNA and its role in the regulation of gene expression make it an attractive drug target in cancer. Further studies are needed to fully understand the role of POLR2J3 in the process of DNA replication and its potential as a drug target.

Protein Name: RNA Polymerase II Subunit J3

Functions: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft (By similarity)

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