POLR2J2: A Potential Drug Target and Biomarker for DNA-Directed RNA Polymorphism

Target Name: POLR2J2

NCBI ID: G246721

POLR2J2: A Potential Drug Target and Biomarker for DNA-Directed RNA Polymorphism

DNA-directed RNA polymerase II (DNA-DPI) is a key enzyme in the replication process of DNA in eukaryotic cells. It is responsible for producing double-stranded RNA from single-stranded DNA templates, thereby maintaining genetic information in the cell. The subunit J2 of DNA-DPI, also known as POLR2J2, plays a crucial role in this process.

Properties

POLR2J2 is a 21 kDa protein that contains 195 amino acid residues. It has a molecular weight of 21 kDa and a calculated pI of 6.5. The protein has a distinct N-terminal and C-terminal region, with a putative N-terminal transmembrane region (TM) and a C-terminal cytoplasmic region (CR). It is a monomer and has a linear structure, with a right-hand orientation of the hands.

Function

POLR2J2 is a key subunit of the DNA-DPI and is required for DNA replication. It functions as a polymerase chain initiation factor, which means it binds to the active site of the complex DNA-DPI and facilitates the initiation of DNA replication. It does this by interacting with the template, the single-stranded DNA, and the primers.

POLR2J2 has been shown to play a role in DNA repair and gene expression. It has been shown to interact with the protein RPA1, which is involved in DNA repair. Additionally, it has been shown to regulate gene expression by activating the p53 gene, thereby influencing cell proliferation and survival.

Drug Targeting

POLR2J2 is a potential drug target due to its involvement in DNA replication and gene expression. Drugs that target this protein have the potential to inhibit DNA replication and/or gene expression, leading to therapeutic benefits in various diseases.

One approach to targeting POLR2J2 is to inhibit its activity with small molecules. A variety of small molecules have been shown to inhibit the activity of POLR2J2, including inhibitors of DNA-DPI (6), RNA-DPI (7), and DNA polymerase I. These inhibitors have been shown to have therapeutic benefits in various diseases, including cancer (9), neurodegenerative diseases (10), and genetic disorders.

Another approach to targeting POLR2J2 is to use antibodies to block its activity. Monoclonal antibodies (MCAs) have been shown to be effective in targeting proteins, including POLR2J2. These antibodies have been shown to be effective in treating various diseases, including cancer (13), autoimmune diseases (14), and genetic disorders.

Biomarker

POLR2J2 can also be used as a biomarker for various diseases. For example, the level of POLR2J2 has been shown to be elevated in various diseases, including cancer (16), neurodegenerative diseases (17), and genetic disorders. Additionally, the activity of POLR2J2 has been shown to be affected by various factors, including temperature, pH, and substrate concentration. These factors can be used to monitor the activity of POLR2J2 and its potential as a biomarker.

Conclusion

POLR2J2 is a protein that plays a crucial role in the DNA replication process. Its activity is affected by various factors, including temperature, pH, and substrate concentration. It is a potential drug target due to its involvement in DNA replication and gene expression, and can also be used as a biomarker for various diseases. Further research is needed to fully understand the role of

Protein Name: RNA Polymerase II Subunit J2

Functions: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft (By similarity)

More Common Targets