PEG-PEG14: A Versatile Tool for Drug Screening and Research (G5195)

Target Name: PEX14

NCBI ID: G5195

PEG-PEG14: A Versatile Tool for Drug Screening and Research

Peptide-conjugated expression elements (PEG-conjugated) are a type of gene expression vector widely used in molecular biology research. By connecting genes to portable tags (such as fluorescein), researchers can track the expression of target genes in real time. . PEG-conjugated expression elements have broad application prospects in the fields of drug screening, drug development, and genetic diagnosis. This article will introduce the PEG-conjugated expression element (PEG-PEG14) and its application in drug screening.

The structure and function of PEG-PEG14

PEG-PEG14 is a PEG-linked DNA fragment consisting of 14 nucleotides. Its structure consists of a 14-amino acid residue protein tail and an end-linked PEG molecule. PEG-PEG14 has the following characteristics:

1. Protein tail: The amino acid tail of PEG-PEG14 is an 伪-helical structure. This structure has good stability and can be expressed in cells for a long time.
2. End-linked PEG molecule: A PEG molecule is connected to the end of PEG-PEG14, making PEG-PEG14 better portability and operability.

Application of PEG-PEG14 in drug screening

Drug screening is a crucial step in the drug development process. By screening proteins with specific functions, researchers can screen out compounds with potential therapeutic value. As a commonly used PEG-linked expression element, PEG-PEG14 is used in drug screening in the following aspects:

1. Screen the target protein: PEG-PEG14 can match the amino acid sequence of a specific target protein to screen out the target protein. This process can be achieved through genetic screening technologies (such as gene chips, liquid chromatography-tandem mass spectrometry).
2. Optimize screening conditions: The PEG molecules connected to the end of PEG-PEG14 can reduce the molecular weight of the protein, thereby reducing the clearance rate of the protein in the cell. At the same time, the stability of PEG-PEG14 makes the protein less likely to denature or degrade during long-term expression. These characteristics make PEG-PEG14 a good drug screening tool.
3. Improve screening efficiency: PEG-PEG14 has good portability and operability. Researchers can improve the efficiency of drug screening by changing the expression amount and screening conditions of PEG-PEG14.

Application of PEG-PEG14 in drug research and development

PEG-PEG14 is widely used in drug research and development, mainly in the following aspects:

1. Drug target screening: PEG-PEG14 can match the amino acid sequence of the drug target protein to screen out compounds with potential therapeutic value. This process can be achieved through high-throughput screening techniques (such as high-throughput sequencing, mass spectrometry-interaction network analysis).
2. Drug target identification: PEG-PEG14 can be used to identify drug targets during drug screening to determine the drug's mechanism of action.
3. Screening of drug side effects: PEG-PEG14 can be used to screen the side effects of drugs. By tracking the expression of target proteins in real time, researchers can discover possible side effects of drugs during treatment, thereby optimizing drug design and reducing adverse drug reactions..

In vivo and in vitro applications of PEG-PEG14

PEG-PEG14 has a wide range of applications both in vivo and in vitro. Here are some main application areas:

1. Drug screening: PEG-PEG14, as a drug screening tool, has been widely used in multiple cancer types, such as lung cancer, breast cancer, prostate cancer, etc.
2. Gene diagnosis: PEG-PEG14 can co-precipitate with proteins in tumor tissues, thereby achieving enrichment of proteins in tumor tissues. This method can be used for early diagnosis and treatment evaluation of tumors.
3. Drug research and development: PEG-PEG14, as a commonly used drug screening tool, plays an important role in the drug research and development process. Through the screening of PEG-PEG14, researchers can quickly screen out compounds with potential therapeutic value, thereby reducing drug development costs.

in conclusion

As a new type of gene expression vector, PEG-PEG14 has broad application prospects in the fields of drug screening, drug research and development, and genetic diagnosis. The protein tail of PEG-PEG14 makes it more portable and maneuverable, while the terminally connected PEG molecules can reduce the clearance rate of the protein within the cell. These characteristics make PEG-PEG14 a good drug screening tool. At the same time, PEG-PEG14 is widely used both in vivo and in vitro, and is a gene expression vector with good development prospects.

Protein Name: Peroxisomal Biogenesis Factor 14

Functions: Component of the PEX13-PEX14 docking complex, a translocon channel that specifically mediates the import of peroxisomal cargo proteins bound to PEX5 receptor (PubMed:9653144, PubMed:24235149, PubMed:28765278). The PEX13-PEX14 docking complex forms a large import pore which can be opened to a diameter of about 9 nm (By similarity). Mechanistically, PEX5 receptor along with cargo proteins associates with the PEX14 subunit of the PEX13-PEX14 docking complex in the cytosol, leading to the insertion of the receptor into the organelle membrane with the concomitant translocation of the cargo into the peroxisome matrix (PubMed:24235149, PubMed:28765278). Plays a key role for peroxisome movement through a direct interaction with tubulin (PubMed:21525035)

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