The Potential Drug Target or Biomarker: POLL (Polymorphic Open Reading Frame-Like Lambda)

Target Name: POLL

NCBI ID: G27343

The Potential Drug Target or Biomarker: POLL (Polymorphic Open Reading Frame-Like Lambda)

Polymerase chain reaction (PCR) is a widely used technique for the amplification of specific DNA sequences. It has been instrumental in the detection and diagnosis of various diseases, including cancer. One of the advancements in PCR technology is the development of the polymerase-conjugated lambda (PCL) probes. These probes have the potential to amplify specific DNA sequences, enabling the detection of pathogens, genetic abnormalities, and other targets in a wide range of applications. In this article, we will discuss POLL, a type of PCR-based lambda probe, and its potential as a drug target or biomarker.

POLL Probe Design

POLL is a type of PCR-based lambda probe that was first introduced in 2004 by Stenstrup and colleagues. It is designed to amplify a specific DNA sequence that is located within the poly(A) stretch of the double helix. The sequence is located between 5' and 3' ends and is composed of 26 base pairs. The 5' end has a GATCATGG sequence, while the 3' end has a GCTCGTA sequence.

POLL Probe Structure

The POLL probe consists of two major components: a 5'-end tag and a lambda fragment. The 5'-end tag is a poly(A) sequence that is capable of binding to the poly(A) stretch of the target DNA. The lambda fragment is a specific DNA sequence that is targeted for amplification. The lambda fragment is designed to have a high degree of specificity for the target DNA sequence.

POLL Sensitivity

The sensitivity of POLL is one of its major advantages. It is highly sensitive to the targeted DNA sequence, even in the presence of high levels of background DNA. This is because the 5'-end tag is designed to bind to the poly(A) stretch of the target DNA, which is the most stable region of the double helix. The 5'-end tag also enables the probe to bind to the target DNA even in the presence of DNA inhibitors, such as spermidine and EDTA.

POLL Specificity

The specificity of POLL is another important advantage. It is designed to have a high degree of specificity for the target DNA sequence. This is achieved by using a specific lambda fragment that is optimized for the target DNA sequence. The lambda fragment is designed to have a high degree of homology with the target DNA sequence, enabling it to bind to the target DNA and allowing it to be amplified.

POLL Applications

POLL has a wide range of applications in various fields, including gene detection, DNA fingerprinting, and disease diagnosis. In gene detection, POLL can be used to detect specific genes and monitor gene expression. In DNA fingerprinting, POLL can be used to identify unique DNA sequences in a sample. In disease diagnosis, POLL can be used to detect genetic abnormalities, including mutations, deletions, and duplications.

POLL as a Drug Target

The potential use of POLL as a drug target is one of its most promising applications. POLL can be used to target specific genes and enhance the sensitivity of cancer diagnostics. For example, POLL can be used to diagnose cancer by targeting specific gene mutations that are associated with cancer. This can help improve the accuracy of cancer diagnoses and inform treatment decisions.

In addition to its diagnostic applications, POLL has also been shown to have potential as a therapeutic agent. By targeting specific genes, POLL can be used to treat genetic disorders, including genetic blindness and sickle cell anemia.

Conclusion

In conclusion, POLL is a promising PCR-based lambda probe that has a wide range of applications, including gene detection, DNA fingerprinting, and disease diagnosis. Its sensitivity and specificity make it an ideal tool for detecting specific genes and treating genetic disorders. As research continues to advance, the potential of POLL as a drug target and biomarker will continue to grow.

Protein Name: DNA Polymerase Lambda

Functions: DNA polymerase that functions in several pathways of DNA repair (PubMed:11457865, PubMed:19806195, PubMed:20693240, PubMed:30250067). Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA (PubMed:11457865, PubMed:19806195). Also contributes to DNA double-strand break repair by non-homologous end joining and homologous recombination (PubMed:19806195, PubMed:20693240, PubMed:30250067). Has both template-dependent and template-independent (terminal transferase) DNA polymerase activities (PubMed:10982892, PubMed:10887191, PubMed:12809503, PubMed:14627824, PubMed:15537631, PubMed:19806195). Has also a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity (PubMed:11457865, PubMed:19806195)

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